Different Selectivity to C18 Phases
The following selectivity test was used to understand the difference in selectivity between a conventional C18 phase, Phenylhexyl and Phenyl phases.
Comparison of Selectivity Between a C18 and Phenylhexyl Phase
Figure 1 compares the selectivity provided by a conventional C18 and Phenylhexyl phase. InertSustain Phenylhexyl show stronger retention of compounds containing nitro groups. Figure 2 compares the separation of analgesic drugs on a conventional C18 and Phenylhexyl phase. Indomethacin was strongly retained on InertSustain Phenylhexyl due to the presence of strong pi-pi interaction resulting in change in elution order with better separation.
Strong Retentivity than other Phenyl Phases
The retentivity of InertSustain Phenylhexyl is stronger comparing with commercially available Phenyl columns.
Sample No.5, n-Amylbenzene was used to determine the hydrophobic property of columns.
Particle Size |
Particle Shape |
Surface Area |
Pore Size |
Pore Volume |
Bonded Group |
End-Capping |
Carbon Load |
pH Range |
3 µm |
Spherical |
350 m2/g |
100Å |
0.85 mL/g |
Phenylhexyl Group |
Complete |
9% |
1 - 10 |
5 µm |