Inertsil ODS-EP offers unique selectivity as hydrogen bonding interactions are provided by polar-embedded functional groups.
Inertsil ODS-EP is recommemded when separation couldn’t be achieved by other separation mechanism such as hydrophobic interactions or pi-pi interactions.
The chromatograms below show the separation of neutral, acid and basic compounds using Inertsil ODS-EP, Inertsil Ph-3 (Phenyl group bonded phase providing pi-pi interactions) and Inertsil C8-3 (pure-alkyl C8 bonded phase providing hydrophobic interactions).
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Excellent peak shape for both acidic and basic compounds
In most of the polar-group-embedded columns on the market, N-containing polar groups are embedded. These phases suffer from unstability in acidic eluents and poor peak shapes due to the adsorption of acidic compounds.
By embedding the proper polar group, Inertsil ODS-EP is able to separate acidic compounds as well as basic compounds with sharp peaks.
In the figure below, separations of acidic compounds in acidic condition, using Inertsil ODS-EP, Inertsil Ph-3 and Inertsil ODS-3, are compared.
Only Inertsil ODS-EP is able to perform clean separation of those structurally similar acidic compounds.
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Stable retention in highly aqueous mobile phases
The embedded polar group helps to wet hydrophobic chains and prevents “phase collaspe”. Therefore Inertsil ODS-EP is compatible with highly aqueous eluents, even with 100% aqueous eluent.
The chromatograms below compare the retentivities of Inertsil ODS-EP and pure alkyl C18 bonded phase. The retentivity of Inertsil ODS-EP is not affected by 100% water eluent.
Particle Size |
Particle Shape |
Surface Area |
Pore Size |
Pore Volume |
Bonded Group |
End-Capping |
Carbon Load |
pH Range |
5 µm |
Spherical |
450 m2/g |
100Å |
1.05 mL/g |
Polar Group embedded C18 |
No |
9% |
2 - 7.5 |
Inertsil® ODS-EP Columns List